Num
1147
Name
FB1027
Alias
pKA10/pet
Source
Karen Arndt
Comments
"using oligo-directed mut, an NdeI site was placed at the spt15 ATG. The 114 3bp NdeI-BamHI frag. was inserted into pET3b, a T7 overexpressor plasmid. **SEE FB1025!** "
Host
BL21(DE3).
Host Markers
pLysS (a plasmid carrying the T7 lysozyme gene; it is CmR) BL21(DE3) is a lmabda lysogen containi
Plasmids
pKA10
Plasmid Markers
AmpR spt15-122 CmR