Num
1145
Name
FB1025
Alias
pKA9
Source
Karen Arndt
Comments
"using oligo-directed mut., an NdeI site was placed at the SPT15 ATG. The 11 43bp NdeI-BamHI frag was inserted into pET3b, a T7 overexpressor plasmid.* *DO NOT GIVE OUT** "
Host
BL21(DE3).
Host Markers
pLysS (is a plasmid carrying the T7 lysozyme gene =it is CmR). BL21(DE 3) is a lamda lysogen contai
Plasmids
pKA9
Plasmid Markers
ampR SPT15+